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prsf 1b s3 m129c  (Addgene inc)


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    Addgene inc prsf 1b s3 m129c
    KEY RESOURCES TABLE
    Prsf 1b S3 M129c, supplied by Addgene inc, used in various techniques. Bioz Stars score: 91/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/prsf 1b s3 m129c/product/Addgene inc
    Average 91 stars, based on 2 article reviews
    prsf 1b s3 m129c - by Bioz Stars, 2026-03
    91/100 stars

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    1) Product Images from "Transient protein-RNA interactions guide nascent ribosomal RNA folding"

    Article Title: Transient protein-RNA interactions guide nascent ribosomal RNA folding

    Journal: Cell

    doi: 10.1016/j.cell.2019.10.035

    KEY RESOURCES TABLE
    Figure Legend Snippet: KEY RESOURCES TABLE

    Techniques Used: Recombinant, Purification, Labeling, Plasmid Preparation, Software



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    (A) Temperature dependence of the long S7-bound lifetime phase shown for Δ270 truncation construct and the full 3’domain RNA in absence of any other r-proteins. The error bars represent the 95 % confidence intervals of the fit. (B) Nomura ribosome assembly map. (C) Experimental setup. (D) The probability of stable S7 incorporation increases with the presence of secondary binding r-proteins; temperature = 35 °C; [Cy5-S7] = 20 nM; [unlabeled r-proteins] = 400 nM, [S15] = 2 μM. (E and F) Simplified single-molecule traces for S7 binding at 35 °C in absence (E) or presence (F) of <t>S9,</t> S13 <t>and</t> <t>S19.</t> (G) Tertiary interactions between H41 and H42 are required for stable S7 binding. The area of the dots is proportional to the populations of the two lifetime phases. The error bars represent the 95 % confidence intervals of the fit. (H) Model on how RNA tertiary interactions and r-proteins progressively stabilize S7. Number of molecules analyzed in (D) (n) = 101, 120, 144, 115, 111, 141, 98, 149, 121, 57, 57. See also Figures S4, S5 and Data S2.
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    KEY RESOURCES TABLE

    Journal: Cell

    Article Title: Transient protein-RNA interactions guide nascent ribosomal RNA folding

    doi: 10.1016/j.cell.2019.10.035

    Figure Lengend Snippet: KEY RESOURCES TABLE

    Article Snippet: Further information and requests for resources and reagents should be directed to and will be fulfilled by the Lead Contact. table ft1 table-wrap mode="anchored" t5 caption a7 REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and Virus Strains E. coli BL21-Gold (DE3) Agilent Cat#230132 E. coli Tuner ™ (DE3) Novagen Cat#70623-3 Chemicals, Peptides, and Recombinant Proteins Protocatechuic acid (PCA) Pacific Biosciences Cat#100-215-400 Protocatechuate-3,4-dioxygenase (PCD) Pacific Biosciences Cat#001-028-310 TSY Pacific Biosciences Cat#100-214-900 Cy5 Maleimide Mono-Reactive Dye 5-Pack GE Healthcare Cat#PA25031 Cy5.5 Sulfo-Cyanine5 maleimide Lumiprobe Cat#13380 Cy3 Maleimide Mono-Reactive Dye 5-Pack GE Healthcare Cat#PA23031 E. coli RNA polymerase holoenzyme NEB Cat#M0551S Biolipidure-203 NOF America Corporation Cat#Biolipidure-203 Biolipidure-206 NOF America Corporation Cat#Biolipidure-206 Purified ribosomal proteins of 16S rRNA 3’domain This paper N/A Oligonucleotides Primers, geneBlocks-based DNA transcription templates, labeled DNA oligonucleotides See Data S1 N/A ACU RNA trinucleotide Dharmacon N/A Recombinant DNA pRSF-1b Novagen Cat#71330-3 pRSF-1b-S2 This paper Addgene Plasmid #128590 pRSF-1b-S3 This paper Addgene Plasmid #128591 pRSF-1b-S7-S83C/delta157-178 This paper Addgene Plasmid #128592 pRSF-1b-S10 This paper Addgene Plasmid #128594 pRSF-1b-S13-C85S/P112C This paper Addgene Plasmid #128595 pRSF-1b-S14 This paper Addgene Plasmid #128596 pRSF-1b-S19-T48C This paper Addgene Plasmid #128597 pET-S9 This paper Addgene Plasmid #132948 pRSF-1b-S3-M129C This paper Addgene Plasmid #132949 pRSF-1b-S15-T79C This paper Addgene Plasmid #133048 Software and Algorithms MATLAB R2015a Matworks Inc http://matlab.com Open in a separate window KEY RESOURCES TABLE

    Techniques: Recombinant, Purification, Labeling, Plasmid Preparation, Software

    (A) Temperature dependence of the long S7-bound lifetime phase shown for Δ270 truncation construct and the full 3’domain RNA in absence of any other r-proteins. The error bars represent the 95 % confidence intervals of the fit. (B) Nomura ribosome assembly map. (C) Experimental setup. (D) The probability of stable S7 incorporation increases with the presence of secondary binding r-proteins; temperature = 35 °C; [Cy5-S7] = 20 nM; [unlabeled r-proteins] = 400 nM, [S15] = 2 μM. (E and F) Simplified single-molecule traces for S7 binding at 35 °C in absence (E) or presence (F) of S9, S13 and S19. (G) Tertiary interactions between H41 and H42 are required for stable S7 binding. The area of the dots is proportional to the populations of the two lifetime phases. The error bars represent the 95 % confidence intervals of the fit. (H) Model on how RNA tertiary interactions and r-proteins progressively stabilize S7. Number of molecules analyzed in (D) (n) = 101, 120, 144, 115, 111, 141, 98, 149, 121, 57, 57. See also Figures S4, S5 and Data S2.

    Journal: Cell

    Article Title: Transient protein-RNA interactions guide nascent ribosomal RNA folding

    doi: 10.1016/j.cell.2019.10.035

    Figure Lengend Snippet: (A) Temperature dependence of the long S7-bound lifetime phase shown for Δ270 truncation construct and the full 3’domain RNA in absence of any other r-proteins. The error bars represent the 95 % confidence intervals of the fit. (B) Nomura ribosome assembly map. (C) Experimental setup. (D) The probability of stable S7 incorporation increases with the presence of secondary binding r-proteins; temperature = 35 °C; [Cy5-S7] = 20 nM; [unlabeled r-proteins] = 400 nM, [S15] = 2 μM. (E and F) Simplified single-molecule traces for S7 binding at 35 °C in absence (E) or presence (F) of S9, S13 and S19. (G) Tertiary interactions between H41 and H42 are required for stable S7 binding. The area of the dots is proportional to the populations of the two lifetime phases. The error bars represent the 95 % confidence intervals of the fit. (H) Model on how RNA tertiary interactions and r-proteins progressively stabilize S7. Number of molecules analyzed in (D) (n) = 101, 120, 144, 115, 111, 141, 98, 149, 121, 57, 57. See also Figures S4, S5 and Data S2.

    Article Snippet: Further information and requests for resources and reagents should be directed to and will be fulfilled by the Lead Contact. table ft1 table-wrap mode="anchored" t5 caption a7 REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and Virus Strains E. coli BL21-Gold (DE3) Agilent Cat#230132 E. coli Tuner ™ (DE3) Novagen Cat#70623-3 Chemicals, Peptides, and Recombinant Proteins Protocatechuic acid (PCA) Pacific Biosciences Cat#100-215-400 Protocatechuate-3,4-dioxygenase (PCD) Pacific Biosciences Cat#001-028-310 TSY Pacific Biosciences Cat#100-214-900 Cy5 Maleimide Mono-Reactive Dye 5-Pack GE Healthcare Cat#PA25031 Cy5.5 Sulfo-Cyanine5 maleimide Lumiprobe Cat#13380 Cy3 Maleimide Mono-Reactive Dye 5-Pack GE Healthcare Cat#PA23031 E. coli RNA polymerase holoenzyme NEB Cat#M0551S Biolipidure-203 NOF America Corporation Cat#Biolipidure-203 Biolipidure-206 NOF America Corporation Cat#Biolipidure-206 Purified ribosomal proteins of 16S rRNA 3’domain This paper N/A Oligonucleotides Primers, geneBlocks-based DNA transcription templates, labeled DNA oligonucleotides See Data S1 N/A ACU RNA trinucleotide Dharmacon N/A Recombinant DNA pRSF-1b Novagen Cat#71330-3 pRSF-1b-S2 This paper Addgene Plasmid #128590 pRSF-1b-S3 This paper Addgene Plasmid #128591 pRSF-1b-S7-S83C/delta157-178 This paper Addgene Plasmid #128592 pRSF-1b-S10 This paper Addgene Plasmid #128594 pRSF-1b-S13-C85S/P112C This paper Addgene Plasmid #128595 pRSF-1b-S14 This paper Addgene Plasmid #128596 pRSF-1b-S19-T48C This paper Addgene Plasmid #128597 pET-S9 This paper Addgene Plasmid #132948 pRSF-1b-S3-M129C This paper Addgene Plasmid #132949 pRSF-1b-S15-T79C This paper Addgene Plasmid #133048 Software and Algorithms MATLAB R2015a Matworks Inc http://matlab.com Open in a separate window KEY RESOURCES TABLE

    Techniques: Construct, Binding Assay

    KEY RESOURCES TABLE

    Journal: Cell

    Article Title: Transient protein-RNA interactions guide nascent ribosomal RNA folding

    doi: 10.1016/j.cell.2019.10.035

    Figure Lengend Snippet: KEY RESOURCES TABLE

    Article Snippet: Further information and requests for resources and reagents should be directed to and will be fulfilled by the Lead Contact. table ft1 table-wrap mode="anchored" t5 caption a7 REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and Virus Strains E. coli BL21-Gold (DE3) Agilent Cat#230132 E. coli Tuner ™ (DE3) Novagen Cat#70623-3 Chemicals, Peptides, and Recombinant Proteins Protocatechuic acid (PCA) Pacific Biosciences Cat#100-215-400 Protocatechuate-3,4-dioxygenase (PCD) Pacific Biosciences Cat#001-028-310 TSY Pacific Biosciences Cat#100-214-900 Cy5 Maleimide Mono-Reactive Dye 5-Pack GE Healthcare Cat#PA25031 Cy5.5 Sulfo-Cyanine5 maleimide Lumiprobe Cat#13380 Cy3 Maleimide Mono-Reactive Dye 5-Pack GE Healthcare Cat#PA23031 E. coli RNA polymerase holoenzyme NEB Cat#M0551S Biolipidure-203 NOF America Corporation Cat#Biolipidure-203 Biolipidure-206 NOF America Corporation Cat#Biolipidure-206 Purified ribosomal proteins of 16S rRNA 3’domain This paper N/A Oligonucleotides Primers, geneBlocks-based DNA transcription templates, labeled DNA oligonucleotides See Data S1 N/A ACU RNA trinucleotide Dharmacon N/A Recombinant DNA pRSF-1b Novagen Cat#71330-3 pRSF-1b-S2 This paper Addgene Plasmid #128590 pRSF-1b-S3 This paper Addgene Plasmid #128591 pRSF-1b-S7-S83C/delta157-178 This paper Addgene Plasmid #128592 pRSF-1b-S10 This paper Addgene Plasmid #128594 pRSF-1b-S13-C85S/P112C This paper Addgene Plasmid #128595 pRSF-1b-S14 This paper Addgene Plasmid #128596 pRSF-1b-S19-T48C This paper Addgene Plasmid #128597 pET-S9 This paper Addgene Plasmid #132948 pRSF-1b-S3-M129C This paper Addgene Plasmid #132949 pRSF-1b-S15-T79C This paper Addgene Plasmid #133048 Software and Algorithms MATLAB R2015a Matworks Inc http://matlab.com Open in a separate window KEY RESOURCES TABLE

    Techniques: Recombinant, Purification, Labeling, Plasmid Preparation, Software